Engineering a diaphorase via directed evolution for enzymatic biofuel cell application
نویسندگان
چکیده
منابع مشابه
Enzymatic Glucose Biofuel Cell and its Application
Biofuel cells have received significant attention in the last few decades due to its potential application as alternative energy sources and advantages over conventional fuel cells. This review summarizes different types of glucose biofuel cells with emphasis on enzymatic glucose biofuel cells. Unlike conventional fuel cells, which use fuel such as ethanol, methanol, formic acid, etc. to genera...
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Enzyme-based biofuel cells (EBFCs) are systems that use a variety of organic compounds to produce electricity through oxido-reductase enzymes, such as oxidase or dehydrogenase as biocatalysts immobilized on electrodes. In this study, a single-chamber EBFC consisting of carbon electrodes that operating at ambient temperature in phosphate buffer, pH 7 is reported. The EBFC anode was based on gluc...
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In the search for improved glucose oxidising enzymes for biofuel cells, a number of Agaricus meleagris (Am) pyranose dehydrogenase mutants (mPDHs) exhibiting different degrees of glycosylation were produced using site-directed mutagenesis and electrochemically characterised. The response of electrodes modified with different mPDHs is compared in a mediated electron transfer mode, where the elec...
متن کاملDirected evolution strategies for improved enzymatic performance
The engineering of enzymes with altered activity, specificity and stability, using directed evolution techniques that mimic evolution on a laboratory timescale, is now well established. However, the general acceptance of these methods as a route to new biocatalysts for organic synthesis requires further improvement of the methods for both ease-of-use and also for obtaining more significant chan...
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A busca contínua por fontes alternativas de energia, imposta por interesses econômicos e ambientais, tem motivado investigações sobre alternativas limpas e eficientes para a produção de energia. As células a combustível são uma estratégia potencialmente eficaz para a conversão de energia. As biocélulas a combustível constituem uma subclasse das células a combustível, que possuem grande potencia...
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ژورنال
عنوان ژورنال: Bioresources and Bioprocessing
سال: 2020
ISSN: 2197-4365
DOI: 10.1186/s40643-020-00311-z